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The plasmid was linearized by the restriction enzyme (Thermo Fisher, Waltham, MA, USA) and used as the template to produce mRNA with T7 High Yield RNA Transcription kit (#DD4202, Vazyme)

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36 A retrospective survey of hepatic copper content in Labrador Retrievers during two time periods (1980-1997 and 1998-2008), revealed significantly higher copper concentrations in the more recent period both in dogs with chronic hepatitis and in control dogs