These findings underscore the need to devise strategies to eradicate intracellular persisters, especially given the limited antibiotic options available for intracellular infections
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Reverse-transcription quantitative PCR Colon tissues were homogenized in 600 l of lysis buffer via bead beating, and RNA was extracted using the GeneJET RNA Purification Kit (Thermo Scientific)
As the diffusion coefficient ( D ) characterizing the random motion (diffusion) of the particles is size-dependent, extended IDPs have smaller D compared to their compact, well-folded counterparts
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