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After a 2D classification with a binned dataset in CRYOSPARC, a homogeneous subset of particles was re-extracted as the native pixel sampling and subjected to Bayesian particle polishing in RELION, followed by another further 2D classification in cryoSPARC

Bonferronis correction was applied to analyses correlating BAL fluid or intracellular GSH, GSSG, and GSH:GSSG to up to 5 gene expression variables or clinical parameters to adjust for multiple comparisons

G., Enzmann, H., Pate, I., and Waechter, F

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So theyre both injected at the same time in a dual-chamber pen