glutathione sulfur amino acid and Acids in Human Health and Disease containing peptide made up of three amino acids Glutamic acid Mode of action of Glutathione

10.3390/cells10092287 9 ChenL.MaY.MaX.LiuL.JvX.LiA.et al (2023)

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Whole cell lysates were prepared by resuspending cell pellets in 1 mL lysis buffer (50 mM Tris pH 7.4 at 4 C, 150 mM NaCl, 10 mM EDTA, 1 mM MgCl 2, 0.5% NP40, 125 U/mL Benzonase, 1x protease inhibitor cocktail (Roche, complete ULTRA mini EDTA free), 0.5 mg/ml Lysozyme, 5 mM DTT), and sonicating with the samples continuously immersed in a circulating 4 C water bath