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However, contrary to expectations, the deletion of the Nfe2l2 gene had a moderate impact on the course of the disease in SOD1 G93A mice and negatively affected only a few Nrf2-regulated antioxidant enzymes, thus suggesting that several Nrf2-target genes can also be regulated independently of Nrf2 in ALS mice [270, 271]

Inflammation biomarkers and risk of all-cause mortality in the Reasons for Geographic And Racial Differences in Stroke cohort

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Another reason could be that your ice is too hard

H.AslundF.BeckwithJ.GeorgiouG

Then the gel was stained with Coomassie Brilliant Blue to visualize the protein bands