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Swab the rubber stopper with an alcohol wipe before every puncture

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We also investigated the histopathological characteristic of each neuronal cell type of the hippocampus

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A80-229AP, Bethyl Laboratories, Inc.) in PBS/T/M was added for 1 h at room temperature, followed by wash and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)

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