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Our study first highlighted the importance of -syn phosphorylation and aggregation in chronic hypoxia-induced cognitive impairment, showing that both -syn knockout and p--syn competing interference resisted chronic hypoxia-induced neuronal injury

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Crystals formed after 12-24 h (SemDAPH) or 5 d (SemDAPH + BR-GBD) were harvested and cryo-protected with mineral oil followed by flash-freezing in liquid nitrogen

The prepared slides were baked, followed by dewaxing, rehydration, staining (employing HE, Safranin O, and immunofluorescence for HIF-1, IL-6, IL-1, TNF-, NF-B(P65)), dehydration, clearing, and mounting

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The process begins in the stratum basale, the deepest layer of the epidermis, where stem cells and progenitor cells are constantly dividing through mitosis