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Pharmacological inhibition of mTORC1 with rapamycin restores the autophagic flux and rescues the defects, pointing to pivotal and complex roles of both mTORC1 and TORC2 in the control of autophagy in the skeletal muscle from young and old individuals (Table 1)

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This avoids repeated needle entry into the vial and reduces freeze-thaw cycling risk

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Sphingolipids, and ceramide, in particular, are found in autophagosomal membranes [63]

J Appl Physiol (1985) 2001;91:534551