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Frequency and administration will vary based on individual needs and goals

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DNA was extracted from the fecal samples using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany)

Data availability All RNA-seq data have been deposited in the GEO and are accessible through GEO Series accession numbers GSE272456 (HCT116) and 810 GSE272391 (AKP organoids) or at RNA-seq data of the clinical cohort have previously been deposited at GSE103479 (ref

Another study found that QUE could activate p53 expression in CO115 and HCT15 CRC cells, rendering these two cells sensitive to 5-FU (30)

Interestingly, mitotically active young muscle has been found to be most responsive to MGF, which argues against a direct effect on quiescent satellite cells [64]