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The values for the free energy of the reaction were calculated using the Minnesota functional of DFT, at the level of theory M06-2X/6311+G(d,p) and SMD, solvation model

17) In order to test, whether oxidation of the active site cysteines over a wide range of GSH/GSSG redox potentials might be common for class I GRXs, Homo sapiens GRX1 (Gene ID: 2745) as a prototypical representative was prepared as a strep-MBP-fusion protein

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They utilized dark pretreatment and found that the expression of both MpGSTZ1 and MpGSTU2 remained unchanged following transfer to the darkness, whereas the expression of MpGSTU1 and MpGSTF1 decreased by ~50 and 75%, respectively, when plants were placed in the dark for 2 h (Dean et al., 2002) found that herbicide safener mefenpyr-diethyl treatment significantly increased GST activity and the expression of HvGST6 (phi class) in dark-grown barley ( Hordeum vulgare L

Perraudin V, Delarue C, Lefebvre H et al