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Measurement of SIRT1 and SIRT3 activities in cultured cells and in mouse tissues Cultured CHO-mCD38 cells, and liver and spleen from treated mice were harvested and homogenized in M-PER Mammalian Protein extraction buffer on ice as described previously 25

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2009 Feb 1;89(2):693S-696S

Protein concentration was determined by colorimetric protein assay

Each batch undergoes analytical verification to confirm molecular identity and purity prior to release

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