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RNA samples extracted from both colon tissue and in vitro culture experiment were reverse transcribed using the qFlex cDNA Synthesis Kit (Quanta Bioscience)

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However, in cultures of the SEV group, the reduction of CCL3 by B12 did not reach statistical significance and intracellular IL-1B levels were very low, close to the detection limit of the technique, suggesting that, in severe COVID-19, leukocytes are more prone to promptly export IL-1B