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4.8
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Description

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OS caused by excessive ROS at physiological levels is likely to affect the functions of hematopoietic stem cells, such as cell growth and self-renewal, which may contribute to defective hematopoiesis

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Molecules 25 (2020)

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04 Longevity Research Peptides and compounds explored in longevity and cellular-aging research contexts

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The dsDNA hairpin was obtained by PCR amplification with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) using Lambda DNA (New England Biolabs) as template and oligonucleotides that include different BsaI restriction sites in each side of the PCR fragment (see Supplementary Tables 3 and 4 for oligonucleotides) followed by purification (QIAGEN) as described previously 47

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