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Insert the needle quickly and decisively at a 90-degree angle into pinched skinhesitation or slow needle entry increases tissue trauma

doi: 10.1038/s41586-021-04359-5 122 RawatAMorrisonBM

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[397] found a significantly increased accumulation of dysfunctional mitochondria in the fibroblasts isolated from SLOS patients under steady-state conditions compared to control cells

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Apart from diet and age, and individual factors the composition of the digestive system microbiota is also influenced by the physiological state and stress