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We therefore present concrete, prioritized experiments (short- and long-term PK, standardized brain AUC measures, chronic biodistribution and microglial phenotyping) that would most efficiently validate nano-design hypotheses and accelerate clinical translation (32, 33)

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Immunofluorescence Microscopy Cells were rinsed two times with PBS followed by 4% paraformaldehyde fixation for 30 min and permeabilizing with 0.2% Triton X-100 for 20 min