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The ERK1/2 mitogen-activated protein kinase pathway as a master regulator of the G1- to S-phase transition

Gateway LR Clonase II was employed to create the binary expression vector 35S pro :tagRFPt-RemA (plasma membrane marker) by recombining the ENTRY vector pENTR_tagRFPt-RemA (Cyprys et al ., 2019), which contains tagRFPt N-terminally fused to the membrane anchor of M

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doi:10.1183/09031936.00209212 86

Interaction with proline 44 leads to precipitation Using solution-state NMR, we were not able to probe the EGCG binding experimentally, since the residues located in the predicted binding site are only poorly visible or cannot be detected at all