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The animals were anesthetized with Isofluran Baxter (IsoFlo, Abbot Laboratories) and intraperitoneally injected with ZRF mixture (Zolazepam 3.8 mg/ml, Tiletamin 3.8 mg/ml Xylazine 0.45 mg/ml and Fentanyl 2.6 g/ml) before transcardial perfusion with 4?C 2% dextran in 0.1 M phosphate buffer (PB) for approximately 30 seconds, immediately followed by 4% formaldehyde (FA) in PB for 10 minutes at the speed of 6 ml/min

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