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This damage was associated with significant lipid peroxidation, which could be mitigated by the antioxidants trolox, butylated hydroxytoluene, N-acetylcysteine, and membrane-permeable iron chelators such as 2,2-dipyridyl, 1,10-phenanthroline, LK614 LK616, and deferoxamine

Biol Trace Elem Res 76(2):161173

MAST4-sh2 slightly affected the ciliary assembly

To purify FBU-His-EEHV-EE2, cell pellets were incubated in lysis buffer (20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 10 mM imidazole, 6 M guanidine hydrochloride (GdmCl)) for 15 min on ice

Factors such as high pressure and speed, during sorting, dye-induced defects, electrical deviation, changes in pH and osmolarity, etc might lead to changes to the sperm plasma membrane, trigger pre capacitation-like changes, cause protamine decondensation and alter sperm DNA quality (68)

Yee, Sook Wah, et al