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The first reaction is catalyzed by -glutamylcysteine synthetase, joining L-glutamate and L-cysteine into -glutamylcysteine

The trials behind oral glutathione used 250mg to 500mg a day not multi-gram "mega doses." One study testing a large 3-gram single dose found it didn't meaningfully raise blood glutathione levels at all

If youd like to see how this process works visually, this short animation provides a clear overview of the methylation cycle and the role of homocysteine below

Additionally, GSH is a main cofactor of GPX4, so GSH regulation can also indirectly modulate GPX4 (Maiorino et al., 2018)

Accordingly, the search for BBB-permeant therapeutic drugs is ongoing