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To preserve neutrophil viability and functional status, the entire procedure from mouse sample collection to completion of flow cytometric sorting was controlled within 6 h

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Role of the gut microbiota in NAFLD Given that the liver is directly linked to the intestine via the portal blood and that the gut microbiome is a major source of acetate, it is not surprising that the gut microbiome plays important roles in NAFLD

Methods Ethics statement Research reported in this study complies with all relevant ethical regulations and was authorized by the Italian Ministry of Health (Authorization n 913/2021-PR) and approved by ethical boards of TIGEM or Institute of Experimental Medicine