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In vitro assessment of hepatoprotective activity using HepG2 cells Cell culture The National Centre for Cell Science in Pune, India, provided the HepG2 cell line, which was cultured in DMEM supplemented with 10% foetal bovine serum, penicillin (100 U/mL), and streptomycin (100 mg/mL) at 37 C in a humidified environment of 5% CO 2

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Reactions were performed in 96-well plates (Corning) at 37 C and monitored turbidimetrically using a Synergy HTX microplate reader (BioTek)

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