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Vitamins in Animal and Human Nutrition (2nd ed.)

Subnormal Concentrations of Serum Cobalamin (Vitamin B12) in Cats with Gastrointestinal Disease

For cycling assays, samples were diluted in 100 mM sodium phosphate buffer (pH 8) in a volume of 100 l per well and added to white 96-well plates

These might be a better option for people with increased nutrient needs or those at a greater risk of deficiency, including individuals with medical conditions that affect absorption

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Briefly, cells were washed twice with PBS, fixed with 10% formalin for 30 min at room temperature, and stained with oil red O working solution ( 0.2% oil red O in 99% isopropanol) for 30 min