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Huh7 and H1299 cells (1 10 4 per well) were sequentially treated with 1.5 M oligomycin, 1 M carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone and 1 M rotenoneantimycin A (both 0.5 M) (all from Sigma)

M.AldanaB

Given that inflammatory insults do not cause depressive symptomology when KP enzymes are blocked, one can surmise that the generation of KP metabolites is a mandatory event preceding the induction of MDD manifestation [117]