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Zyrek AA, Cichon C, Helms S et al (2007) Molecular mechanisms underlying the probiotic effects of Escherichia coli Nissle 1917 involve ZO-2 and PKC

Long-term, high-dose proton pump inhibitors may also affect B12 absorption in some patients

Highly sensitive spatial transcriptomics at near-cellular resolution with slide-seqV2

Those following a vegan diet or with a history of heavy alcohol consumption might need higher B12 supplement dosages

For adults over 65, no dose adjustment is required, but careful monitoring for adverse effects is prudent as older adults often have multiple comorbidities and medications that increase complication risk