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Sartorius) on his-tagged, biotinylated peptide captured on streptavidin beads (PolyAN) and incubated for 90 min with hybridoma supernatant and Atto 488-coupled isotype-specific monoclonal mouse-anti-rat IgG secondary antibodies

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For example, in flies, the lack of any one essential amino acid from the diet produces a strong and specific appetite for proteinaceous or amino acidrich food [56]