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ROS have a significant effect on the sperm plasma membrane and subsequent functional integrity of the sperm resulting in a loss of acrosome reaction [11], the sperm potential for fertilization [12], sperm motility and vitality [13, 14] as well as impair the sperm capacitation [15] (Figure 1)

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miR-93-3p is specifically down-regulated in TCMK-1 cells by the master transcription factor complex CREB1/CRTC2 To explore the mechanism of by which CaOx stimulated the increased expression of miR-93-3p in TCMK-1 cells, we searched the specific transcription factors of mmu-miR-93-3p in the public online database TransmiR (v3.0,cuilab.cn/transmir)

Second, although we included recent human randomized controlled trials (e.g., SYNERGIC, DO-HEALTH, PONDER), the findings are mixed

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