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Notably, after oxidation both products will bind to DNA, which increases their fluorescence

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coded by GSH1 ) catalyses the first and rate-limiting step, during which dipeptide -Glu-Cys is formed from glutamate and cysteine ( GSH2 , which ligates -Glu-Cys with glycine ( S.cerevisiae (coded by GRX1-8 genes)

Similar to normal cells, tumor cells generate ammonium primarily via amino acid deamination, notably through the glutamine cycle, where glutamate dehydrogenase (GDH) catalyzes glutamate deamination to produce free ammonium and -KG