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[1] Whilst B12 supplementation is generally well-tolerated, some individuals report experiencing joint pain or aching after commencing treatment

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The volunteers received an injection of a gel composed of a mixture of cross-linked and non-cross-linked hyaluronic acid, amino acids, glutathione, vitamin B6, and lidocaine, an anaesthetic

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After washing with PBS containing 0.3% Tween 20 (Fisher Scientific, Waltham, MA, United States), the NS3-NS5 interaction was detected by incubating the plates with the anti-NS3 monoclonal antibody (GT2811 clone, Genetex, Irvine, CA, United States) diluted 1:6,000 in PBS + 2% (v/v) FBS (Thermo Fisher Scientific) for 1 h at RT