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In vitro BBB models further show that oligomeric -syn specifically (not monomeric or fibrillar species) disrupts BBB integrity through astrocyte-dependent mechanisms, implicating pathogenic neuronal -syn species as key upstream initiators of vascular fluctuations ( Neuronal -syn pathology drives astrocyte activation through possible release of inflammatory and oxidative stress signals and exposure to extracellular oligomeric species, triggering NF-Bdependent transcription, cytokine production, and in some cases inflammatory senescence (Sorrentino et al., 2017, 2020)

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