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The peptide promotes M2 macrophage polarization, shifting the immune response from a pro-inflammatory to a pro-regenerative state

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Cellular extracts were added in a final volume of 1.0 ml, to 150 mM Tris HCl buffer, pH 8.2, containing 25 mM L-glutamate, 25 mM L--aminobutyrate, 10 mM ATP, 10 mM phosphoenolpyruvate, 30 mM MgCl 2 , 100 mM KCl, 0.2 mM EDTA, 0.42 mM NADH, 7 IU pyruvate kinase, 12 IU lactate dehydrogenase, and the oxidation of NADH was monitored at 340 nm

One of the possible mechanisms reported is plant extracts, and their phytochemicals could increase the levels of GABA in the brain by activating enzymes responsible for the synthesis of GABA