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A single-step second-strand PCR was performed, and the resulting product was purified using a 2:1 ratio of Sera-Mag beads (GE)

The Reason Dead Probiotics Can Still Work After probiotics have been inactivated by heat, the dead bacterial cells release components such as lipoteichoic acids, peptidoglycans, and exopolysaccharide, which can still exert beneficial effects, in particular 17: Modulation of the immune system Neutralization of pathogens Postbiotics is a name you might hear used to describe heat-killed probiotics and their breakdown products and metabolites

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Constitutive activation of PrfA tilts the balance of Listeria monocytogenes fitness towards life within the host versus environmental survival

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