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doi: 10.1016/j.actbio.2022.07.055 203 WangLTongLXiongZChenYZhangPGaoYet al

Even if a peptide receives a favorable recommendation, additional FDA action would still be required before any regulatory changes take effect

To improve the expression, solubilization, and purification of recombinant proteins, it is therefore advised to alter the culture conditions by switching the medium or the temperature, co-expressing chaperones, adding large hydrophilic fusion tags as well as adding sucrose, raffinose, glycine, betaine and sorbitol during growth for enhancing the recombinant protein expression, solubilization and purification [5, 6]

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