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Fatty acid conjugation as used in Semaglutide and Retatrutide promotes reversible albumin binding that shields the peptide from enzymatic degradation and slows renal filtration, extending the half-life from minutes to days

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Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample