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As we further our understanding of parthanatos, new possibilities for therapeutic interventions directed at modulating this cell death pathway are discovered, especially in the context of oxidative stress-associated diseases

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For Ufd1-Bpa experiments, Cdc48 E588Q (500 nM), HA-tagged, Bpa-incorporated Ufd1 (200 nM), Npl4 (500 nM), and Ub(n)-Dy-sfGFP (1 M) were mixed in the reaction buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl 2 , 0.5 mM TCEP, 0.5 mg/mL protease-free BSA) supplemented with 10 mM of ATP

Use small volumes and implement the micro-droplet technique

doi: 10.1021/acssynbio.5c00236 40 ManjulaSBhavanPSKarthikMAnithaDKalpanaRManjulaT