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2.4 Assessment of mitochondrial function and cell viability Mitochondrial function was assessed by measuring adenosine triphosphate (ATP) content and mitochondrial membrane potential using ATP Assay Kit-Luminescence and JC-1 MitoMP Detection Kit, respectively, following the manufacturers instructions (Dojindo Laboratories, Mashiki, Japan)

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Embryonic fibroblasts derived from C57B6 or C57BL/6 mice were immortalized with lentivirus carrying SV40 (Cat

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