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One pathway relies on the vitamin B 12 -dependent methionine synthase and the methyl donor, 5-methyl tetrahydrofolate (a folate derivative), to convert homocysteine back to methionine

This analysis identified that lysine 133 (K133) of IL-1 was targeted for ubiquitylation (marked by a Lys--Gly-Gly (diGly) linkage), while the adjacent serine (S134) was phosphorylated (Fig

Proteins were detected with the indicated antibodies listed in the Antibody table, and blots were imaged using Amersham Imager 680 (GE HealthCare, USA)