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CrossRef Limited Genetic Overlap Between Overt Hashimotos Thyroiditis and Graves Disease in Twins: A Population-based Study Jakob Skov, Jan Calissendorff, Daniel Eriksson, Patrik Magnusson, Olle Kmpe, Sophie Bensing, Ralf Kuja-Halkola The Journal of Clinical Endocrinology & Metabolism.2021

coli BL21 (DE3)/pET-bcfd1 carrying haloacid dehalogenase gene from Bacillus cereus IndB1

The PCR programme used consisted of a first denaturation step at 98 C for 3 min followed by 20 cycles of: (a) 1 min at 98 C, (b) 45 s at 56 C, and (c) 7 min at 72 C

Tessoulin, B

Several other mutations, including p.Ser335Thr and p.Gly350Arg, also localize to the membrane but show no Mn 2+ uptake, suggesting transport-incompetent configurations

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