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Membranes were blocked for 1 h at room temperature in 1% ECL Advance Blocking Reagent (Cytiva) in PBS with 0.01% Tween20 and then incubated overnight at 4C with primary antibodies diluted in PBS supplemented with 5% BSA, 0.05% sodium azide, and 0.01% Tween20

Chest 138 , 929936 (2010)

Publishing Corporation

After mixing with loading buffer, the samples were heated at 100 C for 10 min

doi: 10.1038/s41577-021-00522-1 4 MallahSIGhorabOKAl-SalmiSAbdellatifOSTharmaratnamTIskandarMAet al