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The total protein was extracted from the cells using RIPA buffer (Abcam, Cambridge, UK) containing protease and phosphatase inhibitors (1 g/ml Aprotinin, 1 g/ml Leupeptin, 200 M phenylmethylsulfonyl fluoride (PMSF), 1 mM Na 3 VO 4 )

they want to keep people addicted to nicotine, says Mueller

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