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H., Hiruki, T., Brzezinski, A

three random samples for each experimental condition were used for microarray analysis and all the four samples for quantitative polymerase chain reaction (qPCR) validation

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The ascorbic acid content was measured by following the method of Law et al

Western blot analysis For Western blotting, Y1-BS1 cell lysates were mixed with equal volumes of 5 Laemmli sample buffer [120 mM Tris-HCl, pH 6.8, 2% SDS (w/v), 10% sucrose (w/v), and 1% 2-mercaptoethanol] and subjected to 12% SDS-PAGE

doi: 10.1016/j.taap.2007.01.011 [DOI] [PubMed] [Google Scholar] 123.Cunha-Oliveira T, Rego AC, Garrido J, Borges F, Macedo T, Oliveira CR