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Transmission Electron Microscopy For transmission electron microscopy, the animals were perfused through the heart with PBS buffer (pH 7.4) followed by a mixture of 2.5% glutaraldehyde (GA), 2% PFA, and 0.1 M cacodylate buffer, and then, the brains were isolated and incubated at 4 C for 24 h in the same solution

10.1002/adbi.202200337 168 PfeufferM.AuingerA.BleyU.Kraus-StojanowicI.LaueC.WinklerP.et al (2013)

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Consistency matters more than single applications for visible results

Several other factors may also contribute to raised homocysteine and impaired methylation