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Cells were maintained in a normoxic incubator (37 C, 5% CO, 21% O) or in a hypoxic workstation (Invivo2 400, Ruskinn, UK

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ETC.,, electron transport chain

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Furthermore, most preclinical studies have used short-term high-glucose or db/db mouse models, which fail to recapitulate the chronic, progressive nature of human DN (e.g., long-term fibrosis and comorbidity-associated complications)

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