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Excretion Pathways Limited pharmacokinetic data indicate peptide fragment elimination through standard renal and hepatic routes: Renal excretion of peptide metabolites as primary elimination pathway Hepatic metabolism contributing to peptide degradation Complete elimination within approximately 60 minutes based on half-life calculations No evidence of tissue accumulation or depot formation The dissociation between rapid plasma clearance and prolonged growth hormone elevation demonstrates that sermorelin acts as a secretagogue trigger rather than requiring sustained receptor occupancy

To obtain bacteriological sterility prior to conducting immunopeptidomics experiments, the cell pellet was lysed using 2x lysis buffer and complete protease inhibitor cocktail (Roche) for 30 minutes on ice, with periodic shaking every 10 minutes

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