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This was followed by three brief washes with PBS for 5 min at RT and subsequent incubation in DMEM medium for a total time of 1.5 h at 37 C

A post-Reunion note from Sue Rand Lewis: We took our own advice after the first 61 breakout session and scheduled a Zoom reunion of the 40th and 45th Reunion chairs: Jon Greenleaf in Santa Barbara, CA, Peter (and Elke) Greenberg in Rockville Centre, NY, and Sue Lewis (and Gordon Garrett) in Sarasota, FL