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After blocking, the slides were incubated overnight in rabbit anti-caspase-3 antibody (Catalog No.: 10842-1-AP, Proteintech), anti--synuclein antibody (Catalog No.: 10842-1-AP, Proteintech, China), anti-TH antibody (Catalog No.: 25859-1-AP, Proteintech, China), and anti-NeuN antibody (Catalog No.: A19086, ABclonal, China) diluted 1:100 in blocking solution