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The primary antibodies obtained from Santa Cruz biotechnology (Santa Cruz, CA, USA), which comprised anti-p65 (#sc-8008), anti-COX-2 (#sc-19999), anti-iNOS (#sc-7271), anti-HO-1 (#sc-10789), anti- -actin (#sc-47778), anti-Nrf2 (#sc-518036), and anti-PCNA (anti-proliferating cell nuclear antigen