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On the blot we used primary antibodies against RBD (m RBD 1:2000) and separately against ACE2 (m ACE2 1:1000) with which we detected the specific proteins

The culture medium was replaced with fresh medium every second day without any additional interventions

2.2 The potential molecular mechanisms underlying the therapeutic effects of clemastine The M1 muscarinic acetylcholine receptor (CHRM1) was identified as the major target of clemastine by OPC culture studies