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Deficiency causes megaloblastic anemia and subacute combined degeneration of the spinal cord

Also stated his opinion on every available paper

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Reconstitution & Handling Each 5 mg vial is typically reconstituted with 2 mL of bacteriostatic water, yielding a 2.5 mg/mL working stock, though investigators may adjust volumes based on study design and dosing precision needs

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How 5-Amino-1MQ Inhibits NNMT 5-Amino-1MQ works by binding to the active site of nicotinamide N-methyltransferase (NNMT), a cytosolic enzyme that catalyses the methylation of nicotinamide (a form of vitamin B3) into N-methyl nicotinamide using S-adenosylmethionine (SAM) as the methyl donor