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Discuss For the segmentation of tobacco leaf lesions, traditional methods such as the K-means algorithm used by Teng 19 , the saliency detection method based on seed point selection employed by Xu 20 , and the Otsu algorithm used by Liu et al

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Trap wireworms in pieces of potato scattered around the garden, rotate crops, and plow or cultivate infested soil in late summer or in autumn to kill or expose various insect stages to predators

Amplification and Isolation of Modified TMV Prior to in vitro transcription, the plasmids were linearized by KpnI digestion and then electrophoresed and purified as described above

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