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Methods: Patients with a diagnosis of interstitial cystitis were recruited to this study

Researchers have also uncovered another possible contributor to retinal ganglion cell death: excessive glutamate

After SDS-PAGE analysis on a 15% gel under reducing conditions, the fraction containing the majority of the recombinant proteins was subjected to size-exclusion chromatography by loading the protein extract on an Ultrogel ACA44 (5 75 cm, Biosepra) column equilibrated with a 30 mM Tris-HCl pH 8.0, 1 mM EDTA, and 200 mM NaCl buffer

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